RBS

Part:BBa_M50007:Design

Designed by: Cale Lester, Raquel Freeman, Tristan Yeung   Group: Stanford BIOE44 - S11   (2016-10-31)


U1 Thermosensitive 5'-UTR (includes RBS)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 13
    Illegal PstI site found at 19
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 13
    Illegal PstI site found at 19
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 13
    Illegal PstI site found at 19
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 13
    Illegal PstI site found at 19
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Less than 2kb for DNA 2.0 to synthesize. No Bsa I restriction site sequence. No palindromic repeats longer than 5 bp or repeating nucleotides longer than approximately 10 bp.


Source

Our unique parts are based off of E. coli thermosensors designed by:

Neupert, J., et al. 2008. Design of simple synthetic RNA thermometers for temperature-controlled gene expression in Escherichia coli. Nucleic Acids Research. 36(19): e124.

References